Effects of steam autoclave treatment on Geobacillus stearothermophilus spores

Weibull Model for Inactivation of Geobacillus Stearothermophilus Spores in Superheated Steam

Spores of Geobacillus stearothermophilus at two inoculum levels (3 and 6 log colony forming units per gram, cfu/g) were challenged with superheated steam (SS) at a velocity of 0.35 m/s and temperature of 130, 145, 160, and 175C. Tailing was observed in the survivor curves (plots of survival ratios, log( o N N ) against treatment time) as a monotonic upward concavity. Therefore, a non-linear reg...

Inactivation of Geobacillus stearothermophilus spores by high-pressure carbon dioxide treatment.

High-pressure CO2 treatment has been studied as a promising method for inactivating bacterial spores. In the present study, we compared this method with other sterilization techniques, including heat and pressure treatment. Spores of Bacillus coagulans, Bacillus subtilis, Bacillus cereus, Bacillus licheniformis, and Geobacillus stearothermophilus were subjected to CO2 treatment at 30 MPa and 35...

Formaldehyde gas inactivation of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surface materials.

AIMS To evaluate the decontamination of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surface materials using formaldehyde gas. METHODS AND RESULTS B. anthracis, B. subtilis, and G. stearothermophilus spores were dried on seven types of indoor surfaces and exposed to approx. 1100 ppm formaldehyde gas for 10 h. Formaldehyde exposure significantly de...

Cold plasma technology: bactericidal effects on Geobacillus stearothermophilus and Bacillus cereus microorganisms.

INTRODUCTION Cold plasma, also known as Low Temperature Atmospheric Pressure Plasma (LTAPP) is a novel technology consisting of neutral and charged particles, including free radicals, which can be used to destroy or inactivate microorganisms. Research has been conducted regarding the effect of cold plasma on gram-positive bacteria; however, there is limited research regarding its ability to ina...

Studies on Steam Sterilization and the Effects of Air in the Autoclave.